T1575
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 | Immunoblotting Whole cell extracts of (Lane A) untreated A431 cells, (Lane B) EGF-treated and (Lane C) peroxyvanadate-treated A431cells were separated on SDS-PAGE, blotted with Rabbit Anti-Phosphotyrosine (pY-256) (Cat. No. T1575) at 0.3 μg/mL, and developedusing Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Note: different patterns of protein tyrosine phosphorylation following activation of A431 with EGF versus peroxyvanadate. |
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T1575
Sigma
Anti-Phosphotyrosine (pY-256) antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
| MDL number: | MFCD06411901 |
Description
| Immunogen | phospho-L-tyrosine containing random peptide pY-256, conjugated to KLH. |
| Physical form | Solution in phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
Properties
| antibody form | affinity isolated antibody |
| clone | polyclonal |
| form | buffered aqueous solution |
| application(s) | immunoblotting: 0.5-1.0 μg/mL using cell extract of A431 stimulated by human EGF. |
| indirect immunofluorescence: 5-10 μg/mL using A431 stimulated by human EGF. | |
| storage temp. | −20°C |
Safety
| WGK Germany | NWG |
